![]() ![]() If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. All claims must be made within 30 days following the date of delivery. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. The molecular determinants of specificity and cross. All properties listed are typical characteristics and are not specifications. Cross-reactivity measures the extent to which different antigens appear similar to the immune system. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Please contact a technical service representative for more information. This product is for research use only and is not intended for therapeutic or diagnostic applications. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. Rockland Immunochemical's polyclonal antibody to detect FLAG™ conjugated proteins binds FLAG™ containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGä containing recombinant proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. ![]()
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